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Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
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Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
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Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
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Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
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Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
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Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001
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Image Search Results


Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

Journal: Journal of Nanobiotechnology

Article Title: Mitochondria-targeted nanozyme system for psoriasis treatment

doi: 10.1186/s12951-026-04068-z

Figure Lengend Snippet: Therapeutic effects of CMH@lip@Res-TCeO2 on IMQ-induced psoriasiform skin inflammation. ( A - B ) Macroscopic observation of dorsal skin lesions in mice, evaluating erythema, scaling, and inflammatory infiltration; ( C ) H&E staining to assess histopathological features and changes in epidermal thickness (scale bar: 50 μm); ( D ) Ly6G IF staining to evaluate neutrophil infiltration in skin tissues (scale bar: 50 μm); ( E ) Colorimetric assay to measure MPO activity in skin tissues; ( F - G ) Detection of ROS and MDA levels using fluorescent probes and evaluation of CAT, SOD, and GSH activities using colorimetric assays; ( H ) IHC analysis of Ki67 expression as a proliferation marker in skin tissues (scale bar: 50 μm); ( I ) ELISA quantification of IL-23, IL-1β, IL-17 A, and TNF-α levels in skin homogenates. Each group included six animals. *p < 0.05, **p < 0.01, ***p < 0.001

Article Snippet: Cytokine levels in cell culture supernatants and tissue homogenates were measured using ELISA kits for IL-1β (DY401), IL-6 (M6000B), TNF-α (MTA00B), IL-17 A (DY5390), and IL-23 (M2300) (R&D Systems, USA).

Techniques: Staining, Colorimetric Assay, Activity Assay, Expressing, Marker, Enzyme-linked Immunosorbent Assay